The causative agents of rabies are viruses belonging to the Lyssavirus genus in the family Rhabdoviridae of which the prototypic rabies virus (RABV) is responsible for the vast majority of cases. Following India, China has the second highest number of human cases in the world.
Rabies reportedly causes approximately 55,000 human deaths annually throughout the world, the majority of which occur in Asia. Rabies is a widespread neurological disease and is an ancient fatal encephalitis with nearly 100% mortality. Given the high immunogenicity and good propagation characteristics of the CTNCEC25 strain, it has excellent potential to be a candidate for development into a human rabies vaccine with high safety and quality characteristics for controlling rabies in China. This is the first description of a CTNCEC25 strain that was highly adapted to chick embryo cells, and both its in vitro and in vivo biological properties were characterized.
#RABIES VACCINE SERIAL NUMBER LOOKUP FULL#
The inactivated vaccine induced high antibody responses and provided full protection from an intramuscular challenge in adult mice. The immunogenicity of the CTNCEC25 strain increased with the adaptation process in chick embryo cells and conferred high protective efficacy. A comparison of the structural protein genes of the CTNCEC25 strain and the CTN-1 V strain identified eight amino acid changes in the mature M, G and L proteins. A virus growth curve demonstrated that the CTNCEC25 strain achieved high titers in chick embryo cells and was nonpathogenic to adult mice by intracerebral inoculation. Rabies virus strain CTN-1 V was adapted to chick embryo cells by serial passage to obtain the CTNCEC25 strain.
Although the vaccine strains employed in China include CTN, aG, PM and PV, there are no reports of strains that are adapted to primary chick embryo cells for use in human rabies prevention in China. Currently vaccination is the most effective strategy for preventing rabies, and vaccines are most commonly produced from cultured cells. Rabies virus is the causative agent of rabies, a central nervous system disease that is almost invariably fatal.
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